The sequences from the ColE1 origin primers Employed in two-fragment cloning ended up GGAGCGAACGACCTACACCGAACTGAGATACCTACAGCG and CGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCC. All primers were requested as desalted oligonucleotides and applied without having even further purification. Oxford Academic is household to a wide variety of products and sol